Repair of defined single base-pair mismatches in Escherichia coli
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چکیده
منابع مشابه
Repair of defined single base-pair mismatches in Escherichia coli.
Heteroduplexes with single base-pair mismatches of known sequence were prepared by annealing separated strands of bacteriophage lambda DNA and used to transfect Escherichia coli. Each of the eight possible single base-pair mismatches was constructed. Genetic analysis of the progeny phages obtained from transfected bacteria indicates that the E. coli mismatch repair system does not recognize (or...
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Brutlag, D., Atkinson, M. R., Setlow, P. & Kornberg, A. (1969) Biochem. Biophys. Res. Commun. 37, 982-989 Joyce, C. M. & Grindley, N. D. F. (1983) Proc. Natl. Acad. Sci. U .S .A . 80, 183G1834 Joyce, C. M., Ollis, D. L., Ruch, J., Steitz, T. A,, Knigsber, W. H. & Grindley, N. D. F. (19850) in Protein Structure. Function and Design (Oxender, D. ed.), UCLA Symposia in Molecular and Cellular Biolo...
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Proton nuclear magnetic resonance (NMR) spectroscopy is employed to characterize the kinetics of base-pair opening in a series of 9mer duplexes containing different single base mismatches. The imino protons from the different mismatched, as well as fully matched, duplexes are assigned from the imino-imino region in the WATERGATE NOESY spectra. The exchange kinetics of the imino protons are meas...
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We have used artificially constructed heteroallelic heteroduplex molecules of bacteriophage lambda DNA to transfect Escherichia coli, and E. coli mutants deficient in various functions involved in the adenine methylation-directed mismatch repair system, MutL, MutS, MutH, and lJvrD (MutU). Analysis of the allele content of single infective centers shows that this repair system often acts on seve...
متن کامل1H-NMR Determination of Base Pair Lifetimes in Oligonucleotides Containing Single Base Mismatches
Proton nuclear magnetic resonance (NMR) spectroscopy is employed to characterize the kinetics of base-pair opening in a series of nine-mer duplexes containing different single base mismatches. The imino protons from the different mismatched as well as fully matched duplexes are assigned from the imino-imino region in the WATERGATE NOESY spectra. The exchange kinetics of the imino protons are me...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1985
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.82.2.503